ABSTRACT
Objective To study the chemical constituents from the aerial parts of Hypoestes phyllostachya. Methods The compounds were isolated and purified by silica gel column chromatography, MCI, and HPLC. Their structures were elucidated by spectroscopic analysis. Results Eighteen compounds were isolated from 95% ethanol aqueous extract of H. phyllostachya and the structures were identified as (3R,6R,7E)-3-hydroxy-megastigma-4,7-dien-9-one (1), (3S)-3-hydroxy-β-ionone (2), (3S,5R,6S,7E)-5,6-epoxy-3- hydroxy-megastigma-7-en-9-one (3), grasshopper ketone (4), vomifoliol (5), (+)-dehydrovomifoliol (6), loliolide (7), 2,6-dimethyl- 2E,7-octadien-1,6-diol (8), bifurcanol (9), (12S)-hydroxygeranylgeraniol (10), nectandrin B (11), N-trans-feruloyltyramine (12), syringylethanone (13), paeonol (14), vanillin (15), dehydrozingerone (16), 2-hydroxybenzyl alcohol (17), and (2E)-4-hydroxy-2- hexenoic acid (18). Conclusion All compounds are isolated from this plant for the first time.
ABSTRACT
Objective To study the chemical constituents and anti-neuroinflammatory activity of the caulis of Tinospora sinensis as a "Yao" medicine. Methods The chemical constituents were isolated and purified by silica gel, Sephadex LH-20, ODS column chromatographies, and semi-preparative HPLC. The structures of these compounds were elucidated by extensive spectroscopic analyses and chemical methods. All compounds were evaluated for their anti-neuroinflammatory effect by inhibiting the nitric oxide (NO) production in lipopolysaccharide (LPS)-activated murine BV-2 microglial cells except for compounds 7 and 8. Results Thirteen compounds were isolated from the caulis of T. sinensis. They were identified as tinosposide C (1), tinosposide D (2), seco-isolariciresinol 9-O-β-glucopyranoside (3), (+)-pinoresinol 4-O-β-D-glucopyranoside (4), (+)-syringaresinol (5), tanegoside A (6), (E)-3-[(2,3-trans)-2-(4-hydroxy-3-methoxyphenyl)-3-hydroxymethyl-2,3-dihydrobenzo [b] [1,4]dioxin-6-yl]-N-(4-hydroxyphenethyl) acrylamide (7), thoreliamide B (8), trans-N-p-coumaroyltyramine (9), N-trans-feruloyltyramine (10), N-trans-caffeoyltyramine (11), grossamide K (12), and cis-grossamide K (13), seven of which exhibited significant anti-neuroinflammatory activity with IC50 values ranging from 1.46 to 51.25 μmol/L. Conclusion Compounds 1 and 2 are new compounds and their absolute configurations are confirmed by ECD experiments, compounds 3, 5, 7, 8, and 10-13 are isolated from the plants for the first time. The activity of compounds 9 and 10 is better than positive control minocycline.
ABSTRACT
Objective: To investigate the chemical constituents from Tinosporae Radix (the roots of Tinospora sagittata) and their antiproliferative effects in cancer cells. Methods: The compounds were isolated and purified by means of silica gel, ODS, and Sephadex LH-20 column chromatography. The structures were identified on the basis of physicochemical properties and spectroscopic analysis. The antiproliferative effects in HL-60 and MCF-7 cells were tested by Trypan blue and MTT methods, respectively. Results: Nine compounds were isolated and their structures were identified as tetrahydropalmatine (1), tetrahydrojatrorrhizine (2), jatrorrhizine (3), palmatine (4), neoechinulin A (5), echinuline (6), N-trans-feruloyltyramine (7), uracil (8), and triethylamine hydroiodide (9). Conclusion: Among them, compounds 5, 6, 8 and 9 are isolated from the plants of Tinospora Miers for the first time. Compounds 1, 3-5 and 7 show the moderate antiproliferative effects in HL-60 cells.
ABSTRACT
Objective: To study the chemical constituents from the root barks of Lycium chinense. Methods: The chemical constituents of EtOAc fraction from 95% ethanol extract of L. chinense were isolated and purified by chromatography on silica gel, Sephadex LH-20, and ODS. Their chemical structures were identified on the basis of physicochemical properties and spectroscopic data. Results: Twelve compounds were isolated and identified as N-trans-coumaroyltyramine (1), N-trans-feruloyltyramine (2), dihydro-N-caffeoyltyramine (3), apigenin (4), ferulic acid (5), p-hydroxycinnamic acid (6), 3-hydroxy-1-(4-hydroxyphenyl)-1-propanone (7), 3, 4-dihydroxybenzenepropionic acid (8), 3, 4-dihydroxybenzenepropionic acid methyl ester (9), p-hydroxy-benzoic acid (10), 4-methoxy salicylic acid (11), and nicotinic acid (12). Conclusion: Compounds 8 and 9 are two new natural products, and compounds 1, 6, 11, and 12 are obtained from this plant for the first time.
ABSTRACT
Objective: To study the chemical constituents from the roots and stems of Humulus scandens. Methods: The chromatographies on silica gel column, reverse phase column, and semi-preparative HPLC were used to isolate and purify the constituents and the structures of the compounds were elucidated on the basis of physicochemical properties and spectral data. Results: Ten compounds were obtained from the ethyl acetate fraction of methanol extract in the roots and stems of H. scandens and identitied as N-p-coumaroyltyramine (1), N-trans-feruloyltyramine (2), cosmosiin (3), 3, 3'-dimethoxy-4, 8'-oxyneoligna-9, 4', 7', 9'-tetraol (4), 3-hydroxy-5, 6-epoxy-7-megastigmen-9-one (5), 4, 5-dihydroblumenol (6), scopoletin (7), citruusin C (8), 2-phenylethyl-O-β-D-glucopyranoside (9), and 3-oxo-α-ionol-β-D-glucopyranoside (10). Conclusion: Compounds 2, 4-6, and 8-10 are all obtained from H. scandens for the first time, and compounds 2, 4-6, 9, and 10 are firstly isolated from the plants of this genus. Compounds 2, 4, 7, and 9 show remarkable scavenging activity toward DPPH radical with IC50 values of 0.01, 3.36, 10.55, and 18.15 μg/mL, respectively. The scientific evidence is provided for the development of anti-oxidant and anti-aging aspects of the drugs.
ABSTRACT
Objective: To study the chemical constituents from the roots and rhizomes of Acorus tatarinowii. Methods: Using different chromatographic methods to isolate and purify the constituents of A. tatarinowii, and their structures were identified by physicochemical properties and spectroscopic technology. Results: Thirteen compounds were isolated and identified as fumaric acid (1), nicotinic acid (2), p-hydroxybenzonic acid (3), uracil (4), N-trans-feruloyltyramine (5), thymine (6), variecolorquinone A (7), butanedioic acid (8), tatarol (9), tataroside-12-β-D-glucoside (10), β-sitosterol (11), 2, 5-dimethoxy- benzoquinone (12), and 5-hydroxymethyl-2-furaldehyde (13). Conclusion: Compounds 1-7 are isolated from the plants in Acorus L. for the first time.
ABSTRACT
Objective: To investigate the chemical constituents from the twigs of Myricaria bracteata. Methods: The chemical constituents were isolated and purified by column chromatography. Their structures were identified on the basis of spectroscopic data, such as NMR, MS, and physicochemical properties. Results: Fourteen compounds were isolated from M. bracteata and identified as syringaresinol (1), (-)-lyoniresinol (2), (-)-isolariciresinol (3), N-trans-feruloyltyramine (4), N-trans-feruloyl-3-methoxytyramine (5), N-trans-feruloyl-2'-methoxytyramine (6), kaempferol-3-O-β-D-glucuronic acid methylester (7), quercetin-3-O-β-D-glucuronic acid methylester (8), quercitrin (9), rhamnocitrin (10), gallic acid (11), gallicin (12), E-caffeic acid (13), and E-ferulic acid (14). Conclusion: Compounds 1-6 and 14 are isolated from the plants of this genus for the first time. Compounds 7, 8, 12, and 13 are isolated from the twigs of M. bracteata for the first time.
ABSTRACT
OBJECTIVE: To study the chemical constituents of Alocasia macrorrhiza (L.) Schott. METHODS: Chemical constituents were extracted by organic solvents, isolated and purified by chromatographic techniques with silica gel, polyamide, ODS, Sephadex LH-20, semi-preparative HPLC and recrystallization. Their structures were identified on the basis of physicochemical properties and spectral data analyses of MS and NMR, et al. RESULTS: Nine compounds were isolated from its rhizoma and identified as glycosmisic acid (1), N-trans-feruloyltyramine(2), grossamide(3), protocatechuic acid(4), borneol acetate(5), vanillic acid(6), methyl 4-hydroxybenzoate(7), β-daucosterol(8), and β-sitosterol(9). CONCLUSION: Compounds 1-9 were isolated from Alocasia macrorrhiza for the first time.